- 價格￥1000
- 規(guī)格100ul
- 貨號HKZ150150
- 單位支
- 品牌HaoKebio
產(chǎn)品介紹
產(chǎn)品參數(shù)
資料下載
| 品牌 | 商品編碼 | 商品名稱 | 規(guī)格 | 單位 | 單價 |
|---|
| HaoKebio | HKZ150150 | DOG1兔單克隆抗體 | 100ul | 支 | 1000 |
|---|
DOG1
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50027?Clone# ?BP6032
Predicted?Molecular Wt:
114kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID:??Q5XXA6
Background:
DOG1?is?a?calcium-dependent?chloride?channel?protein?that?is?encoded?by?a?gene?called?TMEM16A (TMEM16?FLJ10261,?ANO1,??ORAOV2, and?AOS2)?located?on?chromosome?11q13.?DOG1?has?many?significant?functions?such?as?regulation?of?the?cholinergic activity of gastrointestinal smooth?muscle and?regulation of?both?the?survival?and?proliferation?of?cells.
DOG1?is detected?in gastrointestinal Cajal cells,?acinic?cells?in?salivary glands?(apical?membraneous?staining,?particularly?in
serous cells),?pancreatic centroacinar cells,?liver cells,?and?epithelium?of?biliary tract,?breast,?stomach,?and?prostate.?More?than?90% of?all?gastrointestinal?stromal?tumors?(GISTs)?are?DOG1 positive,?irrespective?of?kit?mutation?and?CD117?positivity. The
staining?pattern varies from cytoplasmic to?membranous, with?usually strong,?diffuse?intensity.
DOG1?is an?important?marker?in the?identification of GIST together with?CD117,?slightly?more?sensitive?(particularly?in?gastric
GIST without c-kit?mutation) and also?more?specific than?CD117.?DOG1?is?also?useful?in?the?classification?of?salivary?carcinomas,?and?pancreatic and?renal tumors.
Subcellular?location:
Membrane/Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?DOG1?residues?????within?aa1-100 of?DOG1 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of?GIST?????tissue?labelling?DOG1 with?BP6032.?Heat?mediated
antigen?retrieval?was?performed?using
Tris/EDTA?buffer?pH?9.0
1.?Jung?SH, et.al, Gut?Liver.?2011 Jun;5(2):171-80.
2. ?Bergmann?F, et.al,?Hum?Pathol.?2011 Jun;42(6):817-23.
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